
Based on principle of immunochromatographic rapid test (lateral flow) and may be used for qualitative determination of porcine meat antigen in raw and processed minced meat. In immunochromatographic method, the target antigen (porcine heat resistant muscular glycoprotein) bound by highly specific antibodies attached to the test line and colored microparticles. This two-site binding results in formation of immune complex visible as the colored line. It shows negative result in minced meat of following species: beef, lamb, mutton, antelope, horse, chicken, turkey, goose, duck, rabbit, kangaroo.

SAMPLE PREPARATION:
Weigh 0.5 g test portion into a disposable specimen collection tube and record weight to nearest 0.01 g.
Note: use only disposable materials and change with each use.
Add 2.5 mL water, cap the tube and shake vigorously for 15-30 seconds.
Allow to settle. If necessary, test solution can be stored at +2…+8 °C for up to 24 hours.
TEST PROCEDURE
- Bring test strips to room temperature before opening the pouches.
- If the test solution was refrigerated, bring to room temperature.
- Open pouch with a sharp instrument, taking care not to damage the test strip.
- Dip the test strip into the test solution, observing the right direction and depth (not deeper than the colored line marked by arrow signs). Allow the strip to remain in the solution for 5 – 10 seconds.
- Remove the test strip and place onto a CLEAN horizontal surface; do not touch or move the test strip for 5 to 10 minutes.
- Read the test result.
INTERPRETATION OF THE RESULTS
PRECAUTIONS
- Do not use the kit when its pouch is torn, or test strip is broken or damaged. Take care to not damage the test strip when opening the pouch.
- Use the test within 10 minutes after opening the pouch because the test kit is very sensitive to moisture.
- DO NOT TOUCH the reaction membrane.
- All the components of the test kit are disposable; do not reuse. Dispose used components according to regulatory and /or organizational requirements.
- Wear appropriate personal protective equipment (PPE) while handling specimens and test kit components.
STORAGE
- The test strips (dipsticks) should be stored at +18…+25 °С.
EXPIRATION
- The kits is recommended for use within 18 months.
- Do not use the test strips beyond the expiration date.